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Author information Copyright and License information Disclaimer. A nucleotide promoter segment from an IFN-alpha gene renders an unrelated promoter inducible by virus.

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Tissue-specific expression of the human growth hormone gene is conferred in part by the binding of a specific trans-acting factor. Regulation of gene expression by interferons: Open in a separate window.

IRF-1 may also be involved in silencing the function of the SV40 enhancer juxtaposed to the regulatory elements in uninduced cells. Please review our privacy policy.

Human embryonic lung cells HEL-R66 are of monkey origin. Reversible silencing of enhancers by sequences derived from the human IFN-alpha promoter. Support Center Support Center. A rapid and sensitive assay system for bacterial gpt activity in transfected mammalian cells. Sequencing end-labeled DNA with base-specific chemical cleavages.

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Our data suggest that IRF-1 participates in the transient formation of an induction-specific complex es with the regulatory elements. Images in this article Image on p.

Short and long range activation by 460 SV40 enhancer. The human beta-interferon gene enhancer is under negative control.

Heat shock factor is regulated differently in yeast and HeLa cells. In this study, we describe a nuclear factor, termed interferon regulatory factor-1 IRF-1that is involved in the transcription of IFN-beta and possibly other genes.

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Establishment of a permanent cell line HEL-R66 from human embryonic lung cells with high susceptibility to viruses. A nuclear factor that binds to a conserved sequence motif in transcriptional control elements of immunoglobulin genes. This article has been cited by other articles in PMC. Changes of chromatin conformation around mouse interferon-beta gene associated with induction of interferon synthesis. High efficiency DNA-mediated transformation of primate 460.

Biochemistry of interferons and their actions. Transcription of the human interferon-beta IFN-beta gene is induced by a variety of agents such as viruses, dsRNA and some cytokines.

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Interaction of positive and negative regulatory proteins in the galactose regulon of yeast. Regulation of cytokine gene expression.

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BK viral enhancer element and a human cellular homolog. Structure of a chromosomal gene for human interferon beta. Selection for animal cells that express the Escherichia coli gene coding for xanthine-guanine phosphoribosyltransferase. Associated Data Supplementary Materials. Abstract Transcription of the human interferon-beta IFN-beta gene is induced by a variety of agents such as viruses, dsRNA and some cytokines.

Affinity purification of sequence-specific DNA binding proteins.

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The 5′-flanking sequence of human interferon-beta 1 gene is responsible for viral induction of transcription. Intracytoplasmic phosphorylation sites of Tac antigen p55 are not essential for the conformation, function, and regulation 461 the human interleukin 2 receptor.

National Center for Biotechnology InformationIfr. Delimitation and properties of DNA sequences required for the regulated expression of human interferon-beta gene.

Controlled transcription of a human alpha-interferon gene introduced into mouse L cells. Human beta-interferon gene expression is regulated by an inducible enhancer element. Identification of two distinct regulatory regions adjacent to the human beta-interferon gene. Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.